History: Playboy Bunny Girls

The costume itself was conceived by Playboy's director of promotions, Victor Lownes, designed by Zelda Wynn Valdes, and subsequently refined by Hugh Hefner. Originally the ears were taller and the ensemble lacked the trademark bow tie, collar, and cuffs. First unveiled publicly in an early episode of Playboy's Penthouse, it made its formal debut at the opening of the first Playboy Club in Chicago on the evening of February 29, 1960.
Behavior and training
The Playboy Bunnies were waitresses who served drinks at Playboy Clubs. There were different types of Bunnies, including the Door Bunny, Cigarette Bunny, Floor Bunny, Playmate Bunny and the Jet Bunnies (specially selected Bunnies that were trained as flight attendants. They served on the Playboy "Big Bunny" Jet). To become a Bunny, women were first carefully chosen and selected from auditions. Then they underwent thorough and strict training before officially becoming a Bunny. Bunnies were required to be able to identify 143 brands of liquor and know how to garnish 20 cocktail variations. Most dating or mingling with customers was forbidden. Customers were also not allowed to touch the Bunnies, and demerits were given if a Bunny's appearance was not properly organized.